Journal: Journal of Applied Physiology

Article Title: Females display relatively preserved muscle quality compared with males during the onset and early stages of C26-induced cancer cachexia

doi: 10.1152/japplphysiol.00196.2023

Figure Lengend Snippet: Mitochondrial function and markers across time points following C26-tumor implantation. Mitochondrial oxygen consumption measured in permeabilized plantaris and white gastrocnemius muscles of male and female experimental groups normalized to dry fiber weight (O 2 nmol/min/μg). A : state 3 —ADP-induced maximal respiration. B : state 4 —oligomycin-induced proton leakage. Opa1 ( C ) and Bnip3 ( D ) mRNA content of soleus, mixed gastrocnemius, and tibialis anterior muscles for male and female experimental groups. mRNA content is relative to 18S abundance and presented as fold change from phosphate-buffered saline (PBS) control. All values are represented as least square means ± standard error of the mean (SEM). Labeling with different letters represents one-way ANOVA statistical differences of each group comparison at Tukey-adjusted with α value set at P < 0.05 within each sex. No statistically significant differences between groups within each sex are found if the label shares a common letter. If groups are marked by different letters the groups are statistically different from one another. Blue letters represent differences within male groups and pink letters represent differences within female groups. Trend across time points for each sex are represented by *(linear), Ω (quadratic), or ns (no significance). The overall interaction by trend analysis between biological sexes (males and females) is represented by a connector and by *(linear), Ω (quadratic), or ns (no significance). n = 5–16/group. Connecting lines between groups are utilized solely for representational purposes, as each time point was evaluated in a distinct cohort of mice.

Article Snippet: Mitochondrial oxygen consumption was measured polarographically in permeabilized fiber bundles using a respiration chamber (Oxygraph+, Hansatech Instruments, UK) with a method adapted from Min et al. ( 41 ), as described previously ( 5 , 15 ).

Techniques: Tumor Implantation, Muscles, Saline, Control, Labeling, Comparison